Protein purification night at Dan's

Hi everyone.

It's been a while since we've uploaded anything to this blog owing to much changes and projects that we were (and are) working on. We'll be updating the blog shortly with news of what we've been doing all these days, but here's something to tide you guys over.

We held a protein purification workshop a while ago with some of our fellow members. The process itself was a simple exercise using GFP producing plasmid containing E.Coli cells

(prepared before the event itself, since introducing plasmids to E.Coli cells take a while and can't be done in a single night session). Afterward we tried to run the plasmids on gelboxes, one of

our own design and the other one sold commercially.

Here are some of the equipments we used.

While there were a few mistakes during the course of the lab, the purification of the protein itself went along swimmingly. We did have some real issues with running the gelbox though. The trials and tribulations of the commercial and DIY gelbox will be documented at a later date with pictures and possibly a video (when I'm not writing past midnight).